I just had to create a bootable CD/DVD to offline clean a PC of a friend of mine. So I installed and used the Microsoft Malware Removal Starter Kit:
Based on the Windows Preinstallation Environment (Windows PE) kit, great collection for creating a rescue and cleaning disk. It is also very easy to add your own favorite tools as well.
Additional resources for this topic:
The Antivirus Defense-in-Depth Guide:
The Responding to IT Security Incidents page on Microsoft TechNet:
The Windows Security Resource Kit, Second Edition from Microsoft Press: